Miss Brown University Providence, RI, United States
Abstract: Advanced bioprocessing of tissue into individual component cells is required to facilitate direct single-cell analysis of tissues in a variety of diagnostic and therapeutic applications. Herein, a high-throughput device is created that enables robust dissociation of ex vivo and in vitro samples, including tissue biopsies, organoids, and microtissues, into cells using a novel electrical mechanism previously developed by our team. The device was configured to output a 10 V/cm 1 kHz square wave frequency and was tested for its ability to automate conventionally manual processing functions and reduce processing time. Horizontal, parallel plate electrodes were used to process glioblastoma spheroids into cells within 1 minute, followed by automatic centrifugation and sample purification. The first stage of the device performs as well or better at recovering single cells in 1/15th of time required by manual processing, with no decline in cellular viability. This represents the first reported device that is capable of automating all steps of the processing workflow.
