Senior Principal Scientist Sanofi Frankfurt Am Main, Hessen, Germany
Abstract: Cytokines play a crucial role as immune mediators, forming the communication network of the immune system. Therefore, understanding the relative balance of cytokines is essential for unraveling biological pathways. In recent years, a growing array of immunoassay technologies has become available for detecting and quantifying various cytokines. In this study, we present our evaluation data after employing PerkinElmer's HTRF, Promega's Lumit, and Biotechne’s Luminex multiplex immunoassays to measure secreted IL-8 and IL-6 cytokines in cellular supernatants using a 384-well format. We will demonstrate the comparison of robustness and sensitivity by calculating the potencies of molecules that inhibit the secretion of IL-6 and IL-8.
