Dr Goethe University Frankfurt Am Main, Hessen, Germany
Abstract: Autophagy is a cellular process implicated in the renewal of cellular components and the maintenance of cellular hemostasis and therefore associated with various types of diseases. In addition, autophagy belongs to the stress response pathways and is frequently activated by chemical compounds harboring characteristics of cell toxicity. High-throughput screens analyzing autophagy flux are therefore applied in both, the field of compound identification for targeting autophagy and compound characterization for analyzing compound toxicity. We present a live-cell, fluorescent-based screening method for the fast and accurate measurement of autophagy flux and the application for academic research, pharmacological applications, and drug discovery.
