Product Development Scientist SPT Labtech Melbourn, England, United Kingdom
Abstract: In this poster, for the first time, we demonstrate that KAPA Library Quantification Kit protocols can be successfully automated on SPT Labtech’s firefly® liquid handling platform, to increase throughput and efficiency while delivering consistent and reliable results with no detectable well-to-well contamination.
Library quantification is critical to achieving uniform sample pooling and optimal cluster density in next-generation sequencing (NGS) workflows, thereby ensuring the quality and quantity of data output. KAPA Library Quantification Kits contain all the reagents needed for accurate, reliable, and reproducible qPCR-based quantification of NGS libraries for llumina sequencing, and cater to a wide range of library types, concentrations, fragment length distributions and GC content.
firefly from SPT Labtech is a comprehensive benchtop platform that combines all the technologies needed for complete automated qPCR setup, including 96/384-well pipetting and non-contact positive displacement dispensing.
Automating KAPA Library Quantification Kit protocols on firefly allows for the preparation of a 384-well qPCR reaction plate with standards and up to 96 diluted samples in 22 minutes, at least three times faster than when performed by hand. Automating reaction setup also minimizes the inherent risk of human error when pipetting such small volumes across 384-well plates. The resulting qPCR reaction efficiency aligns with expectations for the KAPA Library Quantification Kit. Furthermore, the ΔCq and %CV values obtained are in line with expected values, demonstrating that automating this workflow on firefly provides results comparable to manual workflows in terms of accuracy and consistency.
For Research Use Only. Not for use in diagnostic applications.
