European Sales Manager Molecular Devices Wokingham, Baden-Wurttemberg, Germany
Abstract: This poster presents a novel method for isolating and verifying monoclonal human pluripotent stem cells using an impedance-based single-cell dispenser (DispenCell™) and an automated high-throughput imager (CloneSelect® Imager FL). Our integrated approach significantly improves the efficiency and reliability of monoclonal cell-line development, crucial for bioprocess and drug screening advancements. The process involves dispensing cells into multi-well plates, followed by detailed imaging and growth monitoring. Results demonstrate DispenCell’s gentle cell handling with high viability and CloneSelect® Imager FL’s effectiveness in confirming monoclonality. This innovative technique is a substantial step forward in stem cell research and biotechnological applications.