(1140-A) Morphological profiling datasets based on EU-OPENSCREEN compound libraries

Abstract: Morphological profiling using the Cell Painting assay has emerged as a method for drug discovery research. A multi-stain approach combined with high-content imaging captures morphological changes across various cellular compartments, enabling the rapid identification of the effect of small chemical compounds. We successfully established and validated a Cell Painting assay across four EU-OPENSCREEN partner sites (FMP, IMTM, MEDI, and USC).
As a first step, we have generated a morphological profiling dataset based on 2464 compounds of the EU-OPENSCREEN bioactive library. The EU-OPENSCREEN bioactive library consists of carefully curated and well-annotated compounds with various biological activities including 681 approved drugs and 385 probes. The data was generated over multiple cell lines, namely Hep G2 and U-2 OS, two cell lines that are highly relevant for early drug discovery. Further, we have established and successfully implemented an image and data analysis workflow for rapid analysis and extraction of morphological profiles. This enabled us to assess that the generated data is of good quality, comparable to other established opensource Cell Painting datasets
We are currently applying the established Cell Painting assay on the European Chemical Biology Library (ECBL) with 96,096 structurally highly diverse compounds using the Hep G2 cell line generating one of the largest Cell Painting datasets available to the scientific community. The images and extracted morphological features will be made publicly available, adding to existing morphological profiling datasets, and further facilitating a comprehensive phenotypic characterization of compounds.